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Types of Microscopes and Their functions


This time I will discuss Various kinds of microscopes and microscope parts and functions along, and the parts of microscopes, After my previous membehas How to use Mikroskp

Phase contrast microscopy

The ideal way to observe living things is the natural kadaan: not given color in the state of life, but in normal conditions of life diaphragm bend microscopic (animal tissue or bacteria) ttembus chaya so on each tincram not be observed, this difficulty can be overcome by using fasekontras microscope. The principle of this tool is very complicated .. when the microscope is used nuklus diwwarnai living cells that are not and can not be seen, although it is so because in the cell nucleus, the nucleus is changed a little light through meteri relationships around the core. This relationship is not captured by the human eye dapaat called phase. However, an arrangement of filters and diaphragm in phase contrast microscopy would change the phase difference is a difference in the light of the bright areas and shadows can be captured by the eye * with such a nucleus (and other elements that have so far not seen a visible dapap.

Dark-field microscopy

Gelapdigunakan field microscopy to observe live bacteria, especially bacteria that are so thin that almost approached the compound mikrskop power limit. Dark-field microscopy is different from compound light microscope used only in the case of a special condenser to form a hollow cone beam of light that can be seen. Hollow conical beam of light is reflected at an angle that is smaller than the top of the glass preparation.


Pender Microscope (Microscope Flourenscence

Pender microscope can be used to detect foreign substances or antigens (such as bacteria, rickets, or virus) in the network. In this teknk anttibodi typical protein first isolated from serum or dikonjungsi scene of the series with a fluorescent dye. Because the antibody-antigen reaction was typical besifat, the incident occurred when the antigen fluorescent Akanan the dimaksut there and seen by antibody marked with fluorescent dye.


Ultraviolet Microscope

A variation of the ordinary light microscope is a microscope ultraviolet. Because cahaaya ultraviolet has a shorter wavelength than visible light, ultra violet light for use pecahayaan can improve separation being 2 times more than a regular microscope. Limit of separation and menjadium. Due to ultra violet light can not, see the human nata, a shadow object should be recorded on disk susceptible cahaya9photografi Plate). This microscope uses the power of the lens, and the microscope is too complicated and expensive for the daily work.

Electron Microscope

Is a microscope that can melakuakan peambesaran objects up duajuta times, which uses electro-static and electro maknetik to control lighting and display images and have the ability p [embesaran objects as well as a much better resolution than light microscopy. Electron microscope uses a lot more energy and electromagnetic radiation maknetikmyang shorter than the light microscope.

Various kinds of electron microscopes
Reflection electron microscopy (REM)
Stereo Microscope
Environmental scanning electron microscope (ESEM)
Scanning electron microscope
Transmission electron microscopy (TEM)
Scanning transmission electron microscope (STEM)

Stereo microscope is a type of microscope that can only be used for objects that are relatively large. Stereo microscope has perbesasran 7 to 30 times. The object was observed with a microscope can be seen in three dimensions. The main components of a stereo microscope nearly equal to the light microscope. The lens consists of eyepiece and objective lens. Some differences with the light microscope are:

1. Space stereo microscope lens sharpness is much higher dibandinhkan denan ssehingga light microscope we can see the shape of three-dimensional objects observed

2. The light source comes from above so that the object can be observed thick. Magnification eyepiece biasannya 3 times, so the object prbesaran total at least 30 times. At the bottom there is a table microscope preparations. In the area near there objective Lens lights associated with the transformer. Setting focus objects located next to stalk microscope, while the magnification setting is located above the regulatory Fokos.

Light Microscope

Light microscope has a maximum magnification of 1000 times. Microscopes have less weight and sturdy legs that can stand steady. Light microscope has three dimensions namely lens objective lens, eyepiece and condenser lenses. The objective lens and eyepiece lenses located on either end of the tube on the microscope eyepiece mikroskop.Lensa cast a shadow bias single (monocular) or double (binikuler). Paada lower end of the microscope lens holder obektif are biased lens fitted three or more. Under the microscope tube there is a table which is a preparation microscope. The third system is the condenser lens. Condenser serve to illuminate the object and another microscope lens.

The objective lens work in the formation of the first shadow. This lens determines the structure and the organisms that will determine the split specimen, so as to show the structure of the two adjacent microscopic objects terpisah.Lensa eyepiece, a lens terdpat likrskop the top end of the tube section, adjacent to the eye of the observer. This lens berfugsi to enlarge the image produced by the objective lens. Perbesran shadow formed ranged from 4-25 kali.Lensa condenser serves Padda untukk support a lighting object to be in focus, so that the right will be acquired power pengaturrnnya maximum separation, the two objects into one. Magnification will be less bermanfatjika separate power microscope unfavorable.

In a conventional microscope, light source is barasal of sunlight reflected by a mirror or cukung dataar contained under the condenser In the mirror will direct the light from outside into the condenser. In modern microscopes have dilengkapai lights instead of sunlight.

Microscope parts along with parts and functions

MICROSCOPE TUBE (TUBUS), the tube serves to adjust the focus and relate to the objective lens ocular lens.


REFLECTOR, consists of two types of mirrors are plane mirrors and a concave mirror. Reflector serves to reflect light from the mirror to the object table through a hole in the table contained object and towards the eye of the observer. Flat mirror used when light is needed fulfilled, whereas if less light because it uses a concave mirror to collect light function.

Ocular lens, the lens close to the eye of the lens serves to form a virtual image, upright, and enlarged from the objective lens

Condensers, condenser serves to collect the incoming light, it can swivel up and down.
Micrometers (SMOOTH PLAYER), regulator serves to raise and lower the microscope is slow, and a smaller than makrometer.

Revolver, revolver serves to set the objective lens by the magnification play.

OBJECTIVE LENS, the lens is close to the observed object, the lens forms a real image, upside down, in the view. Where these lenses are regulated by the revolver to determine the magnification of the objective lens.

Diaphragm, more or less serves to regulate the incoming light.
MAKROMETER (WINDING ROUGH), serves for ascending makrometer microscope tube down quickly.

TABLE MICROSCOPE, serves as a place to put the object to be observed.
GLASS CLIPS, clamp serves to clamp the glass that covers the objects that are not easily shifted.
ARM MICROSCOPE, serves as pegangang the microscope.
FEET MICROSCOPE, serves to support or sustain microscope.
JOINT inclination (GOVERNING THE CORNER), to adjust the angle or upright microscope.

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